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Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 162-267
Plasmid#86002Purposebacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53DepositorTypeEmpty backboneUseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6H-SUMO3-ORF24-NTD-Strep
Plasmid#138467PurposeExpresses SUMO3-tagged ORF24-NTD-Strep in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsSUMO3 and Strep-Tag IIExpressionBacterialMutationAmino acids 2-201PromoterT7Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S
Plasmid#40394DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161,C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28a-His6-SUMO-RABIF
Plasmid#135556PurposeExpress human RABIF in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertRABIF (RABIF Human)
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Lys17Ala
Plasmid#208171PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLys17Ala substitutionPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-SynCas.v10 bacteria
Plasmid#208594PurposeProduction and purification of catalytically active SynCas.v10DepositorInsertSynCas.v10
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria
Plasmid#208595PurposeProduction and purification of catalytically inactive SynCas.v10DepositorInsertdSynCas.v10
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-SynCas.v12 bacteria
Plasmid#208596PurposeProduction and purification of catalytically active SynCas.v12DepositorInsertSynCas.v12
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria
Plasmid#208597PurposeProduction and purification of catalytically inactive SynCas.v12DepositorInsertdSynCas.v12
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F
Plasmid#200877PurposeZooTsy_TnsF expression construct for protein purification.DepositorInsertZooTsy_TnsF
UseTagsExpressionBacterialMutationTnsF-Y584FPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiA-FLAG
Plasmid#229220PurposeFor expression of T. elongatus KaiA proteinDepositorInsertkaiA (kaiA T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutationPromoterAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
small ubiquitin-like modifier 3 (SUMO3)
Plasmid#221053PurposeExpression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoterDepositorInsertSUMO 3 (SUMO3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
small ubiquitin-like modifier 1 (SUMO1)
Plasmid#221052PurposeExpression of small ubiquitin-like modifier 1 (SUMO1) protein under T7 promoterDepositorInsertSUMO 1 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)
Plasmid#231379PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Human, Synthetic)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56A, R68A, R97A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiB-FLAG
Plasmid#229221PurposeFor expression of T. elongatus KaiB proteinDepositorInsertkaiB (kaiB T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-SeKaiB-K25C-FLAG
Plasmid#229225PurposeFor expression of S. elongatus KaiB protein mutantDepositorInsertkaiB (kaiB S. elongatus)
UseTagsFLAG and SUMOExpressionBacterialMutationK25CPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-sumo
Plasmid#226107PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assaysDepositorInsertDELE1 (DELE1 Human)
UseTagsSumoExpressionMammalianMutationencodes only residues 142-515 of DELE1 (represent…PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Tyr33Ala
Plasmid#208170PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorInsertsyt1 (96-421) I367C (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationC277A; I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorInsertsyt7 (136-403) (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
UseTagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
UseTagsSUMOExpressionBacterialMutationY96C, C139A, C141SPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHis-SUMOExpressionBacterialMutationΔ37PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorInsertRab10 (RAB10 Human)
UseTagsHis-SUMOExpressionBacterialMutationQ68LPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET1-DHDDS/Sumo-NgBR
Plasmid#203162PurposeExpression of human cis-prenyltransferase complex for purificationDepositorUseTagsHis6-SUMOExpressionBacterialMutationtruncationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO HNRNPA1 dHexa LCD
Plasmid#197014PurposeE. coli expression vectorDepositorInsertHNRNPA1
UseTags6His-SUMOExpressionBacterialMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse ZNG1-METAP1 fusion
Plasmid#197398PurposeExpresses a SUMO-tagged fusion of ZNG1, residues 10-30 and mouse METAP1, residues 1-59DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CylK-Strep
Plasmid#185962PurposeExpresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparationDepositorInsertCylK
UseTagsHis6-SumoExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only