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pZF-BCD

Plasmid

#127472

Purpose

coding sequence for synthetic zinc finger ZF-BCD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-AA-GCN

Plasmid

#127479

Purpose

coding sequence for synthetic zinc finger ZF-AA-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AA fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BAB

Plasmid

#127469

Purpose

coding sequence for synthetic zinc finger ZF-BAB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BAB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BDC

Plasmid

#127473

Purpose

coding sequence for synthetic zinc finger ZF-BDC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDC synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BDD

Plasmid

#127474

Purpose

coding sequence for synthetic zinc finger ZF-BDD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ADD

Plasmid

#127468

Purpose

coding sequence for synthetic zinc finger ZF-ADD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ADB

Plasmid

#127467

Purpose

coding sequence for synthetic zinc finger ZF-ADB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNP159

Plasmid

#106361

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

ttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-GFP-HA no-pA

Plasmid

#82589

Purpose

3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AN+

Plasmid

#78240

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS-

Plasmid

#78239

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS+

Plasmid

#78236

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS+

Plasmid

#78252

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSABAD14-111

Plasmid

#61824

Purpose

NpuDnaB(delta283)dN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaB(delta283)dN11 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD518

Plasmid

#46374

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

TvoVMA intein, inactive

Tags

H6-GB1 and SH3

Expression

Bacterial

Mutation

N186A, T+1A mutations, inactive

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCK01_PalkS_alkS

Plasmid

#166502

Purpose

Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

alkS

Use

Synthetic Biology

Expression

Bacterial

Mutation

L401G

Promoter

PalkS

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-ABE8e_gRNA

Plasmid

#202559

Purpose

P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Article

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_YafQ/DinJ

Plasmid

#229148

Purpose

Toxin-Antitoxin (Dinj/YafQ)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, DinJ/YafQ

Expression

Bacterial

Promoter

PtlpA (mCherry), DinJ/YafQ (Native)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-BlastR

Plasmid

#194059

Purpose

Mammalian expression of evoCjCas9 nuclease

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_Txe/Axe

Plasmid

#229147

Purpose

Toxin-Antitoxin (Txe/Axe)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, Txe/Axe

Expression

Bacterial

Promoter

PtlpA (mCherry), Txe/Axe (Native)

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Mouse ZP3 promoter (JD#445)

Plasmid

#14634

Depositor

Jurrien Dean

Article

Rankin et al Development. 1998 Jul . 125(13):2415-24.

Insert

ZP3 promoter (Zp3 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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FL-mCherry

Plasmid

#78862

Purpose

DULIP negative control (prey) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RIPK4_pCSdest

Plasmid

#53897

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RIPK4 (RIPK4 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p2T-CMV-evoCjCas9-TadCDd-BlastR

Plasmid

#202558

Purpose

Mammalian expression of evoCjCas9 TadCDd

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM552

Plasmid

#154131

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

wt bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1alphaFEM1CalphaFLAG

Plasmid

#236462

Purpose

transient overexpression of FEM1C in mammalian cells

Depositor

Michele Pagano

Article

Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

Insert

FEM1C (FEM1C Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ptRNA100

Plasmid

#154133

Purpose

Expresses a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

tRNAs operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEG R2-L3 #BGV193

Plasmid

#48952

Purpose

For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Expression

Plant

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pDH42(CUG/A)

Plasmid

#154132

Purpose

Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

engineered bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
PA-RL-mCherry

Plasmid

#78861

Purpose

DULIP negative control (bait) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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