We narrowed to 842 results for: BirA

1. pSLIK BirA* 3xFLAG-CSE1L zeo

   Plasmid

   #192334

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1L

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*-3xFLAG-CSE1L (CSE1L Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

   Plasmid

   #172583

   Purpose

   For transient expression of C-terminal BirA-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

   Plasmid

   #172582

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD2

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 rtTA BirA eIF4G +MIC

   Plasmid

   #158790

   Purpose

   pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (with microexon)
   

   Use

   Tags

   3xFlag and BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3/flag-pBirA(1-K283)-FRB

   Plasmid

   #154451

   Purpose

   expresses flag-pBirA(1-K283)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-K283)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti PGK BirA Hygro (w874-1)

   Plasmid

   #29649

   Depositor

   Eric Campeau

   Insert

   BirA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-sox10-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80063

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Sox10 promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

   Plasmid

   #86885

   Purpose

   C-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   BirA* (AA141-321)- HA- NIPP1]
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 141-321

   Promoter

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXL-CAG-mBirA272-NRX3b D137A

    Plasmid

    #43922

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    BirA272-NRX3b (birA Synthetic, Human)
    

    Use

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    D137A

    Promoter

    CAG

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid

    #174692

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

    Plasmid

    #120562

    Purpose

    Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

    Plasmid

    #86886

    Purpose

    N-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    PP1g - FLAG - BirA (AA2-140)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 2-140

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

    Plasmid

    #120561

    Purpose

    Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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