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Showing: 241 - 260 of 605 results

  2. pcDNA3 RL3m6L2

    Plasmid
    #109366
    Purpose
    Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag
    Depositor
    Robert Lucas
    Article
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Insert
    Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
    Use
    Tags
    1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109366-plasmid-map-sequence-id-210142

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  4. DG SaCas9 GFP V3

    Plasmid
    #226961
    Purpose
    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226961-plasmid-map-sequence-id-450824

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  5. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-450823

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  6. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

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  7. Pdg459-eSp(1.1) V3

    Plasmid
    #226965
    Purpose
    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226965-plasmid-map-sequence-id-450820

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  8. DG SaCas9 puro V3

    Plasmid
    #226960
    Purpose
    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226960-plasmid-map-sequence-id-450810

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  14. pCEP4 TAPBPR-TM-TN6

    Plasmid
    #178650
    Purpose
    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)
    Depositor
    Erik Procko
    Article
    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038
    Insert
    TAPBPR (TAPBPL Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    E205K, R207E, Q209S, Q272S, switched transmembran…
    Promoter
    CMV
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    178650-plasmid-map-sequence-id-354770

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  16. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  17. ETV1A-c014

    Plasmid
    #53655
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    ETV1A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. ACVR1A-c076

    Plasmid
    #53691
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACVR1A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. CCNKA-c001

    Plasmid
    #53644
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CCNKA
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. KCNK10C-c011

    Plasmid
    #53639
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCNK10C
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 605 results