We narrowed to 7,616 results for: Pet2

1. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-pduCDE

   Plasmid

   #110138

   Purpose

   Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

   Insert

   pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24b-BG1.3

   Plasmid

   #199437

   Purpose

   expresses BAmGreen1.3 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen1.3
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24b-BR1.4

   Plasmid

   #199436

   Purpose

   expresses BAmRed1.4 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMRed1.4
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-BE1

   Plasmid

   #73018

   Purpose

   Expresses BE1 with N-terminal His6 tag in E. Coli

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-MKH8SUMO

   Plasmid

   #79526

   Purpose

   Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pET28a-Rx3(A7)

   Plasmid

   #35525

   Depositor

   Robert Cohen

   Article

   Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

   Insert

   His6-Rx3(A7)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Changed G2 to P

   Promoter

   T7

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nampt-PET28a

   Plasmid

   #25630

   Depositor

   Cynthia Wolberger

   Article

   Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

   Insert

   Nampt (Nampt Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pET29a-hNABP1

    Plasmid

    #128306

    Purpose

    Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-csgB

    Plasmid

    #89582

    Purpose

    Express csgB curli protein with GS linker and Histaq

    Depositor

    Urartu Seker

    Article

    Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

    Insert

    csgB (csgB )
    

    Use

    Tags

    3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Ec.coaA

    Plasmid

    #50386

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss

    Article

    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

    Insert

    PanK
    

    Use

    Tags

    6XHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23b-NTF2

    Plasmid

    #108918

    Purpose

    Expresses NTF2

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    NTF2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET26b-Granzyme A

    Plasmid

    #8823

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A (GZMA Human)
    

    Use

    Tags

    (His)6 and Enterokinase site

    Expression

    Bacterial

    Mutation

    it mature enzyme.

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BglB in pET29b(+)

    Plasmid

    #55773

    Purpose

    Expresses beta-glucosidase

    Depositor

    Justin Siegel

    Article

    Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

    Insert

    Beta-glucosidase B (bglB Synthetic)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a Ov-iPGM

    Plasmid

    #180245

    Purpose

    Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    Onchocerca volvulus iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a Wb-iPGM

    Plasmid

    #180246

    Purpose

    Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    Wuchereria bancrofti iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge