We narrowed to 3,869 results for: SPL;

1. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

   Plasmid

   #69587

   Purpose

   Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53-L344P (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   p53 L344P mutation that prevents dimerization and…

   Promoter

   EF1alpha

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

   Plasmid

   #184335

   Purpose

   Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha-GPI (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-Split-Broccoli-Toehold-Switch

   Plasmid

   #87316

   Purpose

   Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

   Plasmid

   #73266

   Purpose

   Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Use

   Tags

   His/V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

   Plasmid

   #69582

   Purpose

   Expresses p53 tagged with mKate2 and split N-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - N term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-FLEX-splitTVA-EGFP

   Plasmid

   #59332

   Purpose

   Expresses splitTVA-P2A-EGFP

   Depositor

   Ian Wickersham

   Insert

   splitTVA950-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Laccase2 MCS-WT Split GFP

   Plasmid

   #69891

   Purpose

   Expresses a GFP circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   GFP, laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNAS1b split mCherry NEDD4-2 WW2

   Plasmid

   #111883

   Purpose

   Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Use

   Tags

   Split mCherry system: NEDD4-2 WW2 fused to C-term…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNAS1B split mCherry N-MESVD/TRAP(RK)-C

   Plasmid

   #61967

   Purpose

   Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-MESVD
   TRAP(RK)-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid

    #69586

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNAS1b split mCherry NEDD4 WW2

    Plasmid

    #111882

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: NEDD4 WW2 fused to C-termin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ER-mit RspA-splitFAST (lentiviral plasmid)

    Plasmid

    #233604

    Purpose

    Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-splitFAST
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/SV40

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTub-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199210

    Purpose

    For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid

    #223503

    Purpose

    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisplay-PGD2-1.1-IRES-mcherry

    Plasmid

    #214763

    Purpose

    Express GRAB-PGD2-1.1 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDisplay-PGD2-1.0-IRES-mcherry

    Plasmid

    #214762

    Purpose

    Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.0 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASt-Xbp1-HA-GFP-3'UTR Spl

    Plasmid

    #201246

    Purpose

    Xbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced form

    Depositor

    Pedro Domingos

    Article

    Cairrao et al Nat Commun. 2022 Mar 24;13(1):1587. doi:

    Insert

    dXbp1 (Xbp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge