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pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA)

Plasmid

#212348

Purpose

Encodes SARS-CoV-2 spike E484K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N439K (AAA)

Plasmid

#212332

Purpose

Encodes SARS-CoV-2 spike N439K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N439K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N439K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

Plasmid

#148158

Purpose

Insect Expression of Dm4E-T_1-511-FWKtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-FWKtoAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X

Plasmid

#147985

Purpose

Insect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147875

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W

Plasmid

#147859

Purpose

Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Plasmid

#137116

Purpose

Protease Switch Clone ID: 1-C9

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W

Plasmid

#147886

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

Plasmid

#147887

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N679K (AAA) P681H (CAC)

Plasmid

#212480

Purpose

Encodes SARS-CoV-2 spike N679K, P681H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N679K, P681H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N679K, P681H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA)

Plasmid

#212470

Purpose

Encodes SARS-CoV-2 spike S477N, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L452R (CGT) T478K (AAA)

Plasmid

#212437

Purpose

Encodes SARS-CoV-2 spike L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417T (ACG) E484K (AAA)

Plasmid

#212382

Purpose

Encodes SARS-CoV-2 spike E484K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) E484K (AAA)

Plasmid

#212352

Purpose

Encodes SARS-CoV-2 spike K417N, E4848K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, E4848K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, E4848K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA) N501Y (TAT)

Plasmid

#212354

Purpose

Encodes SARS-CoV-2 spike E484K, N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K, N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K, N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

Plasmid

#212472

Purpose

Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K, E484A

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA)

Plasmid

#212438

Purpose

Encodes SARS-CoV-2 spike K417N, L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pET151-Vps4(101-437)-Hcp1

Plasmid

#87737

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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