We narrowed to 6,478 results for: nls

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TANGO1S-mScarlet-i

Plasmid

#165461

Purpose

Encodes TANGO1S tagged with mScarlet-i

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

TANGO1S

Tags

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p330 VQR

Plasmid

#101730

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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vHB8

Plasmid

#193454

Purpose

Expression of GFP-Cas9-NLS and gRNA in A. castellanii

Depositor

Chantal Abergel

Article

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

GFP-Cas9-NLS

Use

CRISPR

Tags

GFP

Promoter

GAPDH

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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px459 VRER

Plasmid

#101716

Purpose

sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-N-PrLD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKA-207I10

Plasmid

#79845

Purpose

Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pTiger-OptoSOS

Plasmid

#86439

Purpose

Single-construct blue light optogenetic Ras activator for use in Drosophila

Depositor

Jared Toettcher

Article

Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

Insert

OptoSOS

Tags

tagRFP

Expression

Insect

Promoter

Gal4-UAS

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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yWL234 MCP-GFP-PABPC1*

Plasmid

#197054

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-PABPC1*

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc- GFP-Flag

Plasmid

#82471

Purpose

Expresses myc-GFP-Flag

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-GFP-Flag

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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px458 EQR

Plasmid

#101731

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMB66

Plasmid

#47946

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

hsp-16.48

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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JAB2240

Plasmid

#190359

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-NLS-19Sterm

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP S2TAG

Plasmid

#68296

Purpose

S2TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP S2TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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