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Plasmid#179384PurposeLentiviral vector for expression of Human Transferrin receptor-1 fused mAppleDepositorInsertTransferrin receptor 1 - mApple (TFRC Synthetic, Human)
UseLentiviralTagsmAppleExpressionMammalianMutationPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(N3)-PP1beta
Plasmid#44223DepositorInsertPP1B (PPP1CB Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
Plasmid#139961PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanicaDepositorInsertConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV/hSyn-SCLM
Plasmid#216760PurposeAAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleonDepositorInsertSuperClomeleon
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterhSyn (human synapsin I)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mhYFP
Plasmid#186530PurposeVisualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)DepositorInsertMonomeric hyperfolder YFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a-DIO-SCLM
Plasmid#216759PurposeAAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoterDepositorInsertSuperClomeleon
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterEF1a (human elongation factor 1 alpha)Available sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-LSSmGFP
Plasmid#186540PurposeVisualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.DepositorInsertLSSmGFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12CTermTAG
Plasmid#125267PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertCTermTAG
UseTagsHis-TagExpressionMutationPromoterTrcAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYI012
Plasmid#170841PurposepGH3.17::GUSPlus::tOCSDepositorInsertGH3.17p
UseTagsExpressionPlantMutationPromoterGH3.17pAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Asp73TAG
Plasmid#125266PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Asp73TAG
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-GFP-HiBit
Plasmid#170989PurposeFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-hfYFP
Plasmid#186520PurposeVisualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)DepositorInsertHyperfolder YFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-R9-GFP-HiBit
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CMV-SCLM
Plasmid#216758PurposeAAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleonDepositorInsertSuperClomeleon followed by WPRE and human growth hormone polyA terminator
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCMV (cytomegalovirus)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
S. rosetta Col+ cDNA amplified library
Pooled Library#62844Purposeamplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensisDepositorExpressionBacterialSpeciesSalpingoeca rosettaAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only