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We narrowed to 3,220 results for: sv40

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Showing: 2601 - 2620 of 3220 results

  2. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  4. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

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  7. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  9. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  10. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  13. Anti-DYKDDDDK [M2] (Recombinant Antibody)

    Antibody
    #194502-rAb
    Purpose
    Anti-DYKDDDDK recombinant mouse monoclonal antibody; binds to FLAG tag sequence.
    Depositor
    Melina Fan
    Recommended Applications
    Western Blot
    Reactivity
    Synthetic
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available Since
    Feb. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  14. Anti-c-Myc [9E10] (Recombinant Antibody)

    Antibody
    #188875-rAb
    Purpose
    Anti-c-Myc recombinant mouse monoclonal antibody
    Depositor
    Melina Fan
    Recommended Applications
    Western Blot
    Reactivity
    Human
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available Since
    Sept. 2, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  15. Anti-c-Myc [9E10] - Chimeric (Recombinant Antibody)

    Antibody
    #194521-rAb
    Purpose
    Anti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a goat IgG3 Fc.
    Depositor
    Melina Fan
    Recommended Applications
    Western Blot
    Reactivity
    Human
    Isotype
    IgG3
    Trial Size
    Available to purchase
    Available Since
    Feb. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  16. Anti-c-Myc [9E10] - Chimeric (Recombinant Antibody)

    Antibody
    #194520-rAb
    Purpose
    Anti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.
    Depositor
    Melina Fan
    Recommended Applications
    Western Blot
    Reactivity
    Human
    Source Species
    Chicken
    Isotype
    IgY
    Trial Size
    Available to purchase
    Available Since
    Feb. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  17. Anti-DYKDDDDK [M2] - Chimeric (Recombinant Antibody)

    Antibody
    #198002-rAb
    Purpose
    Anti-DYKDDDDK [M2] chimeric recombinant antibody; binds to FLAG tag sequence. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.
    Depositor
    Melina Fan
    Recommended Applications
    Immunocytochemistry and Western Blot
    Reactivity
    Synthetic
    Source Species
    Rabbit
    Isotype
    IgG
    Trial Size
    Available to purchase
    Available Since
    April 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  18. Anti-c-Myc [9E10] - Chimeric (Recombinant Antibody)

    Antibody
    #194540-rAb
    Purpose
    Anti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.
    Depositor
    Melina Fan
    Recommended Applications
    Immunocytochemistry and Western Blot
    Reactivity
    Human
    Source Species
    Rabbit
    Isotype
    IgG
    Trial Size
    Available to purchase
    Available Since
    Feb. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  19. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  20. pPD8_33

    Plasmid
    #1468
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1468-plasmid-map-sequence-id-79

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Showing: 2601 - 2620 of 3220 results