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pYTRW17K_6G5

Plasmid

#177285

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW16K_1G5

Plasmid

#177286

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW13K_3G5

Plasmid

#177287

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW18K_3T5

Plasmid

#177288

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW28K_0Ti1

Plasmid

#177290

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD130_hIrg1_M154I_pvp008

Plasmid

#239117

Purpose

hACOD1 with M154I mutation in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag-II

Expression

Bacterial

Mutation

M154I

Promoter

T7

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD131_hIrg1_M154I_pCMV6

Plasmid

#239120

Purpose

hACOD1 with M154I mutation in mammalian expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

Myc and FLAG

Expression

Mammalian

Mutation

M154I

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-RspA-CFAST

Plasmid

#233607

Purpose

Expression of RspA-CFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPOLR2D

Plasmid

#229022

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

POLR2D gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgCh2

Plasmid

#229023

Purpose

Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgFOCAD #2

Plasmid

#229024

Purpose

Expression of a EnAsCas12a guide targeting FOCAD

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

FOCAD gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051d_sgCiChr2-2

Plasmid

#228940

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTag4C_Nrg1-GV-2HA

Plasmid

#227104

Purpose

Nrg1 protein for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Nrg1-GV (Nrg1 Mouse)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0410-luc2

Plasmid

#227121

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0410

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0410 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0432-luc2

Plasmid

#227124

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0432

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0432 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0434-luc2

Plasmid

#227125

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0434

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0434 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0407-luc2

Plasmid

#227119

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0407

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0407 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0436-luc2

Plasmid

#227126

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0436

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0436 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge