We narrowed to 5,546 results for: KRAS

1. pTE4560

   Plasmid

   #107526

   Purpose

   Expresses As crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   mCherry
   

   Expression

   Mammalian

   Promoter

   CBh and human U6

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-miR-125b-1

   Plasmid

   #58990

   Purpose

   MSCV based expression vector - co-expresses mCherry and human miR-125b-1

   Depositor

   David Baltimore

   Article

   So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

   Inserts

   mCherry fluorescent protein
   miR-125b-1 (MIR125B1 Human)
   

   Use

   Retroviral; Mscv-based retroviral system

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-Integrin-Beta1-N-18

   Plasmid

   #55423

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

   Plasmid

   #124870

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221-TAZ CA

   Plasmid

   #79504

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3xFLAG

   Mutation

   S89A

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-TEV-FLAG

   Plasmid

   #105783

   Purpose

   Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p221- purVSG117UTR

   Plasmid

   #59732

   Purpose

   Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

   Depositor

   Gloria Rudenko

   Article

   Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

   Insert

   VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
   

   Use

   For homologous recombination into vsg221 expressi…

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-AIRAPL-Strep-HA

   Plasmid

   #113489

   Purpose

   Expression of human AIRAPL with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   AIRAPL (ZFAND2B Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15542_nCBE-SuperFi-Cas9

    Plasmid

    #184372

    Purpose

    Mammalian expression of SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15544_nABE-SuperFi-Cas9

    Plasmid

    #184374

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BRCA1-(1316-1538)

    Plasmid

    #35079

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1316-1538

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

14. BRCA1-(498-663)

    Plasmid

    #35070

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-663

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5FRT/TO-UBXD5-Strep-HA

    Plasmid

    #113493

    Purpose

    Expression of human UBXD5 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD5 (UBXN11 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BRCA1-(540-758)

    Plasmid

    #35071

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 540-758

    Promoter

    T7

    Available Since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Tags

    Strep-Strep

    Expression

    Mammalian

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEX-SaCas9-U6-sgGabrg3

    Plasmid

    #124858

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

    Plasmid

    #124863

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

    Plasmid

    #124872

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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