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tdEos-Farnesyl-5

Plasmid

#57613

Purpose

Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Farnesyl (HRAS Human)

Tags

tdEos

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69894

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

Plasmid

#69887

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Mutation

F169L in ZKSCAN1

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69896

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 neo

Plasmid

#72569

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69886

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-AtGCS1-HAP2-V5-H2B-GFP

Plasmid

#240495

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

AtGCS1 (HAP2 Mustard Weed)

Tags

GFPnes and V5

Expression

Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

Plasmid

#196235

Purpose

EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(K35E/Y145R)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K35E and Y145R

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-TEX264(28-313aa)

Plasmid

#227714

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

TEX264(28-313aa) (TEX264 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-FAM134C(250-466aa)

Plasmid

#227715

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FAM134C(250-466aa) (RETREG3 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_AtGCS1-HAP2-V5_H2B-RFP

Plasmid

#240500

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

AtGCS1 (HAP2 Mustard Weed)

Tags

H2B-RFP and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Plasmid

#239699

Purpose

BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2

Plasmid

#223757

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI3

Plasmid

#223763

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI3 (WDR45B Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI3

Plasmid

#223768

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI3 (WDR45B Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d

Plasmid

#223725

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_WIPI2d-TEV-GST

Plasmid

#223799

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Tags

TEV-GST

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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