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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,412 results for: Library

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Showing: 2641 - 2660 of 3412 results

  3. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  4. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  7. pRegev_V2

    Plasmid
    #204144
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V2 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Truncated CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204144-plasmid-map-sequence-id-405477

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  8. pRegev_V3

    Plasmid
    #204145
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V3 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Truncated CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204145-plasmid-map-sequence-id-405464

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  11. pGJJ055

    Plasmid
    #183622
    Purpose
    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183622-plasmid-map-sequence-id-361477

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  16. pRegev_D1

    Plasmid
    #204178
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D1 in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Truncated CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204178-plasmid-map-sequence-id-405445

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  17. pRegev_D4

    Plasmid
    #204184
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    D4 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Truncated CAG
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204184-plasmid-map-sequence-id-405407

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  18. pRegev_D2

    Plasmid
    #204180
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D2 in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    61C>G mutation
    Promoter
    Truncated CAG
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204180-plasmid-map-sequence-id-405406

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Showing: 2641 - 2660 of 3412 results