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pZA16mflon

Plasmid

#75439

Purpose

Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Dual-recombinase-controller

Plasmid

#44456

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

pTEt-Bxb1, pBAD-TP901

Use

Synthetic Biology

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCis_2+7+8+5

Plasmid

#60589

Purpose

a 4-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+7+8+5

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72103

Plasmid

#40785

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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p5419

Plasmid

#168186

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::tag-rfp-T PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pSEL2

Plasmid

#23012

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

Kanamycin resistance

Tags

6 His Tag and Xpress epitope

Expression

Bacterial

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
p15A_J23108_SpydCas9_CmR

Plasmid

#119163

Purpose

Expresses S. pyogenes dCas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

dCas9 from S. pyogenes

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p5426

Plasmid

#168187

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::dsred PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEG3a

Plasmid

#31398

Depositor

Kenn Gerdes

Article

Ebersbach et al Mol Microbiol. 2008 May . 68(3):720-35.

Insert

zapB (zapB E. coli)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only
pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCis_7+8

Plasmid

#60587

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_araC_Para

Plasmid

#243911

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC and ParaBAD

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pMut12_apo64C

Plasmid

#247072

Purpose

L-arabinose inducible rAPOBEC-XTEN64-CasC(Cas7)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

rAPOBEC-XTEN64-CasC-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-C1

Plasmid

#127395

Purpose

Expresses amcyan fluorescent gene

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1531

Plasmid

#226637

Purpose

This plasmid is for correcting the nonsense mutation in the nrsB gene of several ICP1 isolates.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Insert

Upstream sequence of nrsB (only 338bp total)

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pVY-16 TC-TEM1

Plasmid

#194630

Purpose

antibiotic resistance enzyme with tetracysteine tag for visualization of enzyme

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-TEM1

Tags

MGCCPGCCGGA

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Portal_prophage_Desulfurococcales_archaeon

Plasmid

#188816

Purpose

Portal protein from Desulfurococcales archaeon ex4484_217_2 prophage

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Portal

Expression

Bacterial

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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