We narrowed to 6,235 results for: nls

1. SepOTSι

   Plasmid

   #68290

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EFSep21, 2xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. SepOTSη

   Plasmid

   #68288

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 2xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SepOTSζ

   Plasmid

   #68287

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 2xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SepOTSδ

   Plasmid

   #68285

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 1xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xFNLDD/pMXs-I2

   Plasmid

   #48880

   Purpose

   Expresses 3xFNLDD in mammalian cells

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

   Insert

   3xFNLDD
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sgf73(1-106)/PET32a

   Plasmid

   #26962

   Depositor

   Cynthia Wolberger

   Article

   Samara et al Science. 2010 May 21. 328(5981):1025-9.

   Insert

   Sgf73(1-106) (SGF73 Budding Yeast)
   

   Tags

   His6 and Trx

   Expression

   Bacterial

   Mutation

   with TEV site to cleave the N-terminal his+Trx tag

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pT2iApowriter2(#336)

   Plasmid

   #184104

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2iU6Apowriter2N(#355)

   Plasmid

   #184106

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis + in vitro trans…

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi+SP6

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT22Apowriter2(#340)

   Plasmid

   #184105

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-BAT-RED

    Plasmid

    #20674

    Depositor

    Tannishtha Reya

    Insert

    Wnt reporter dsRED
    

    Tags

    NLS

    Expression

    Bacterial

    Available Since

    May 26, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-Cdc42-2G

    Plasmid

    #68813

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx4-Cdc42-2G

    Plasmid

    #68814

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Tags

    His and Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR1326

    Plasmid

    #111131

    Purpose

    Expresses UBE2T.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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