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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 2641 - 2660 of 6562 results

  8. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

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  9. pCM39

    Plasmid
    #261126
    Purpose
    For C-terminal a tagging protein of interest with VN173 (split-YFP) and mTagBFP expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    myo-3
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261126-plasmid-map-sequence-id-520972

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  10. pCM38

    Plasmid
    #261125
    Purpose
    For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    myo-3
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261125-plasmid-map-sequence-id-520103

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  11. pCM64

    Plasmid
    #261134
    Purpose
    For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261134-plasmid-map-sequence-id-520101

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  12. pCM70

    Plasmid
    #261135
    Purpose
    For N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261135-plasmid-map-sequence-id-520100

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  13. pCM34

    Plasmid
    #261121
    Purpose
    For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    rgef-1p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    rgef-1
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261121-plasmid-map-sequence-id-520093

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  14. deltaVP1 + VP2C-MIOX

    Plasmid
    #166680
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166680-plasmid-map-sequence-id-329927

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Showing: 2641 - 2660 of 6562 results