We narrowed to 36,577 results for: Spr

1. pYPQ290-E848A

   Plasmid

   #129675

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Tags

   E848A; AacCas12b is rice codon optimized

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ291-D573A

   Plasmid

   #129676

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D573A; BthCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ291-D951A

   Plasmid

   #129677

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D951A; BthCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ291-E827A

   Plasmid

   #129678

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   E827A; BthCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ292-D977A

   Plasmid

   #129680

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D977A; AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ292-E848A

   Plasmid

   #129681

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   E848A; AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS424-ysg(NT)

   Plasmid

   #138258

   Purpose

   Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1C3-FeGFP

   Plasmid

   #138261

   Purpose

   Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB1C3-ysg(NT)

    Plasmid

    #138263

    Purpose

    Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PX330_ZBTB7B_2

    Plasmid

    #135747

    Purpose

    Encodes gRNA for 3' target of human ZBTB7B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB7B gRNA (ZBTB7B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX330_ZBTB7B_1

    Plasmid

    #135746

    Purpose

    Encodes gRNA for 3' target of human ZBTB7B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB7B gRNA (ZBTB7B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid

    #117140

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Insert

    gDmap1 v1 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPOR0SP0012

    Plasmid

    #117533

    Purpose

    Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    eSaCas9 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R499A,Q500K,R654A,G655R

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR0SP0019

    Plasmid

    #117529

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.1 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPOR0SP0018

    Plasmid

    #117528

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.1

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.1 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPOR0SP0017

    Plasmid

    #117527

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPOR0SP0015

    Plasmid

    #117525

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-KA (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K855A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPOR0SP0001

    Plasmid

    #117523

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-DE (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D1135E

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge