We narrowed to 10,294 results for: otos

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1193

Plasmid

#167614

Purpose

Express TonB H20N

Depositor

Kathleen Postle

Article

Swayne et al J Bacteriol. 2011 Aug;193(15):3693-701.

Insert

tonB H20N

Expression

Bacterial

Mutation

tonB with H20N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1064

Plasmid

#167607

Purpose

Express ExbD D25N protein

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbD D25N

Expression

Bacterial

Mutation

exbD with D25N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRF028d

Plasmid

#156349

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Six5 pGWB402

Plasmid

#118013

Purpose

expresses Six5 (without signal peptide). Used in combination with Avr2-GFP pZK538(#118011) for visualization of cell-to-cell movement of Avr2

Depositor

FLW Takken

Article

et al Bio-protocol 8(24): e3114.

Insert

dspSix5

Expression

Bacterial and Plant

Promoter

35S

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF H407A H409A H448A

Plasmid

#139566

Purpose

expressed SznF H407A H409A H448A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H407A H409A H448A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMX234

Plasmid

#23006

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

mRFP-CenpB

Tags

mRFP1.0

Expression

Mammalian

Mutation

deleted CenpB amino acids 169-606, T145S

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pITRSF4

Plasmid

#89699

Purpose

For production of GFP with "GLP" N-tag and "TRNGL" for C-tag for cyclisation control.

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

SMT3_GLP_H6_GFP_TRNGL

Tags

SMT3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJTRSF50

Plasmid

#89700

Purpose

For production of GFP with "GLP" N-tag and "TRNCL" for C-tag for cyclisation control.

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

SMT3_GLP_H6_GFP_TRNCL

Tags

SMT3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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recoverin1a

Plasmid

#83811

Purpose

express zebrafish recoverin1a

Depositor

Stephan Neuhauss

Article

Zang et al Open Biol. 2015 Aug;5(8). pii: 150086. d

Insert

recoverin1a (rcvrna Zebrafish)

Expression

Bacterial

Mutation

*(see below)

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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recoverin2a

Plasmid

#83813

Purpose

express zebrafish recoverin2a

Depositor

Stephan Neuhauss

Article

Zang et al Open Biol. 2015 Aug;5(8). pii: 150086. d

Insert

recoverin2a (rcvrn2 Zebrafish)

Expression

Bacterial

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSKB3.TM1468

Plasmid

#11431

Depositor

Sung-Hou Kim

Article

Schulze-Gahmen et al Proteins. 2003 Mar 1. 50(4):526-30.

Insert

fatty acid binding protein

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/tdTomato-nat

Plasmid

#120326

Purpose

Bacterial tdTomato labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

tdTomato

Expression

Bacterial

Promoter

proD

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-Sh_ble

Plasmid

#120329

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comment below)

Promoter

(None)

Availability

Academic Institutions and Nonprofits only

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pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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