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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTW2065

Plasmid

#236574

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_A

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLZRS-IRES-deltaNGFR

Plasmid

#72930

Purpose

retroviral expression with FACS-selectable marker (extracellular part of NGFR)

Depositor

Esther van de Vosse

Article

van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTW2157

Plasmid

#236581

Purpose

ISYmu1 CHLl1 gRNA7 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2156

Plasmid

#236580

Purpose

ISYmu1 CHLl1 gRNA6 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2153

Plasmid

#236577

Purpose

ISYmu1 CHLl1 gRNA3 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2159

Plasmid

#236582

Purpose

ISYmu1 CHLl1 gRNA9 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2155

Plasmid

#236579

Purpose

ISYmu1 CHLl1 gRNA5 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-GFP

Plasmid

#97151

Purpose

label apical epithelial cells in zebrafish

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

AcGFP

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-CypA

Plasmid

#132934

Purpose

Lentivector for CypA knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

CypA shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102G

Plasmid

#112160

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge