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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,130 results for: LEA

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Showing: 2701 - 2720 of 12130 results

  2. GST-TRIM24+TEV

    Plasmid
    #28147
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Tags
    GST and TEV cleavage site
    Expression
    Mammalian
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. pBAF

    Plasmid
    #104152
    Purpose
    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
    Depositor
    Robert Craigie
    Article
    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95
    Insert
    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    Tags
    N-terminal His tag with thrombin cleavage site
    Expression
    Bacterial
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    104152-plasmid-map-sequence-id-201395

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  9. pResQ shFEN3 3XF-FEN1 wt

    Plasmid
    #17752
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  11. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  14. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25158-plasmid-map-sequence-id-472908

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  17. pAAV-hSyn-GRAB_OT1.0 (AAV1)

    Viral Prep
    #185386-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3-ABKAR

    Plasmid
    #140051
    Purpose
    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.
    Depositor
    Jin Zhang
    Article
    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4
    Insert
    ABKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140051-plasmid-map-sequence-id-307466

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  20. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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Showing: 2701 - 2720 of 12130 results