Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,310 results for: LEA

Clear keywords
Showing: 2701 - 2720 of 12310 results

  1. pICE-HA-MRE11-H63S

    Plasmid
    #82035
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H63S MRE11 mutant
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82035-plasmid-map-sequence-id-155388

    Enlarge

  5. pcDNA3-TRAIL-R3

    Plasmid
    #61384
    Purpose
    Expresses TRAIL receptor 3
    Depositor
    Francis Chan
    Publication
    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRAIL receptor 3 (TNFRSF10C Human)
    Tags
    HA tag and TNF receptor leader peptide
    Expression
    Mammalian
    Mutation
    TRAIL-R3 leader peptide (aa1-29) replaced with TN…
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174079-plasmid-map-sequence-id-341130

    Enlarge

  7. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Publication
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    His-NavMs, Voltage gated sodium channel
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100004-plasmid-map-sequence-id-192452

    Enlarge

  10. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Publication
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

    Enlarge

  11. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral prep
    #112677-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3-InPGrp-Kras

    Plasmid
    #236001
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Publication
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InPGrp-Kras (Cyth3 Synthetic, Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236001-plasmid-map-sequence-id-473749

    Enlarge

  16. pcDNA3-Venus-173-N-ORAI1

    Plasmid
    #87618
    Purpose
    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.
    Depositor
    Jin Zhang
    Publication
    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus173-N-ORAI1 (ORAI1 Human, Synthetic)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87618-plasmid-map-sequence-id-176683

    Enlarge

  17. GST-BHMT-I/G

    Plasmid
    #104442
    Purpose
    Autophagy cargo assay
    Depositor
    Carol Mercer
    Publication
    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BHMT (BHMT Human)
    Tags
    GFP-myc GFP is from Aequorea victoria and GST
    Expression
    Mammalian
    Mutation
    N43A in GST (in a potential KFERQ-like sequence).…
    Promoter
    CMV
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104442-plasmid-map-sequence-id-200428

    Enlarge

  19. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73209-plasmid-map-sequence-id-139734

    Enlarge

Showing: 2701 - 2720 of 12310 results