Addgene

We narrowed to 5,155 results for: PEP

Showing: 2701 - 2720 of 5155 results

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pHH0103_KPNA2_Arm

Plasmid

#109866

Purpose

Protein expression and purification of KPNA2_Arm

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KPNA2_Arm (KPNA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABCE1_ABC-tran

Plasmid

#109853

Purpose

Protein expression and purification of ABCE1_ABC-tran

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ABCE1_ABC-tran (ABCE1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF2S3_GTP-EFTU

Plasmid

#109829

Purpose

Protein expression and purification of EIF2S3_GTP-EFTU

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF2S3_GTP-EFTU (EIF2S3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARPC5_P16-Arc

Plasmid

#109802

Purpose

Protein expression and purification of ARPC5_P16-Arc

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARPC5_P16-Arc (ARPC5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA2_ThiF

Plasmid

#109787

Purpose

Protein expression and purification of UBA2_ThiF

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA2_ThiF (UBA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARPC3_P21-Arc

Plasmid

#109793

Purpose

Protein expression and purification of ARPC3_P21-Arc

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARPC3_P21-Arc (ARPC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

Plasmid

#155136

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

D243G/L244G/F245G/M247G/S248G/M249G/A250G

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBB0238-TEV-His12

Plasmid

#137038

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66635.2

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ATP5

Plasmid

#109857

Purpose

Protein expression and purification of ATP5

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ATP5 (ATP5J Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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csd6_4-330/pET28b(+) N-tag

Plasmid

#71351

Purpose

csd6, construct:4-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only