We narrowed to 5,546 results for: KRAS

1. pET15b p97-His D592Amb

   Plasmid

   #169022

   Purpose

   Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   D592TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ARB366

   Plasmid

   #124049

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-TIRAP-cGASΔN-HA

   Plasmid

   #130921

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   TIRAP-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the N ter…

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-1 p37delta2-81

   Plasmid

   #113502

   Purpose

   Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   delta 2-81

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL His-I3

   Plasmid

   #113513

   Purpose

   Expression of His-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Tags

   6*His

   Expression

   Insect

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Thy-C73A-HA

   Plasmid

   #111542

   Purpose

   For expression of human KCNB1 in mouse brain

   Depositor

   Federico Sesti

   Article

   Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

   Insert

   thy1.2-C73A-HA (KCNB1 Human)
   

   Use

   Mouse Targeting

   Tags

   HA

   Mutation

   C73A

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

   Plasmid

   #58490

   Purpose

   Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

   Insert

   Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Glutamine

   Promoter

   Ubiquitin

   Available Since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Hmga2 shMut wt

   Plasmid

   #52036

   Purpose

   encodes shMut HMGA2

   Depositor

   Julian Downward

   Article

   Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Expression

   Mammalian

   Mutation

   shRNA binding site mutated

   Promoter

   CMV

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

9. BRCA1-(219-408)

   Plasmid

   #35067

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 219-408

   Promoter

   T7

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Ef1a-fDIO-tdTomato (AAV1)

    Viral Prep

    #128434-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-tdTomato (#128434). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-tdTomato plasmid DNA. EF1a-driven, Flp-dependent expression of tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato (Flp-dependent)

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Ef1a-fDIO-GCaMP6f (AAV1)

    Viral Prep

    #128315-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-GCaMP6f (#128315). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6f plasmid DNA. EF1a-driven, Flp-dependent expression of calcium sensor GCaMP6f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

    Viral Prep

    #171027-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV1)

    Viral Prep

    #171027-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

14. ALL-PAM sensor Library

    Pooled Library

    #179219

    Purpose

    10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences.

    Depositor

    Lukas Dow

    Article

    Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

    Expression

    Mammalian

    Use

    Lentiviral

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

15. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL-mouse cGAS #1-gRNA-Cas9-Puro

    Plasmid

    #186890

    Purpose

    gRNA targeting mouse cGAS (with Cas9 insert)

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Cas9 (Cgas Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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