We narrowed to 23,310 results for: promoter

1. pTAT5

   Plasmid

   #112593

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6xHis-thioredoxin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAT3

   Plasmid

   #112584

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. P0050::kaiBC

   Plasmid

   #101828

   Purpose

   drives expression of kaiBC from an rpaA-independent promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kanamycin casette and p0050 promoter
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. P0050::rpaA

   Plasmid

   #101830

   Purpose

   drives expression of rpaA from an rpaA-independent promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA with p0050 promoter (+ gentamicin resistance casette)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ptrc::gnd

   Plasmid

   #101844

   Purpose

   drives expression of gnd using Ptrc promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   Kan casette followed by ptrc promoter
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP

   Plasmid

   #80912

   Purpose

   Reporter plasmid (mCherry and GFP)

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P5G6T and PM,5G6T

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD3-proD/tdTomato-nat

   Plasmid

   #120326

   Purpose

   Bacterial tdTomato labeling

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4-eIF3B

   Plasmid

   #133308

   Purpose

   Expression of luciferase driven by eIF3B promoter region

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter (EIF3B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   eIF3B

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4-eIF3B MBE2 mutant

   Plasmid

   #133309

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE2 mutant (EIF3B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   gccacatgcacc changed to gcAaAaAAcacc

   Promoter

   eIF3B

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-CBP FL

    Plasmid

    #179550

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. hTph2p-Luc

    Plasmid

    #223526

    Purpose

    Firefly-luciferase reporter expression driven by human Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Tph2 promoter (TPH2 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    hTph2 promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGL4.11-COL1A1WT

    Plasmid

    #230915

    Purpose

    Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    luciferase - Luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-flag-hKDM4A-polyA

    Plasmid

    #101051

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. hGFAP-Cre

    Plasmid

    #40591

    Purpose

    Mouse astrocyte expression of Cre

    Depositor

    Albee Messing

    Article

    Zhuo et al Genesis. 2001 Oct;31(2):85-94.

    Insert

    Cre recombinase (GFAP Human, Phage, Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    MP-1 fragment (mouse protamine 1 intron and polya…

    Expression

    Mammalian

    Mutation

    Construct contains a nuclear-targeted Cre recombi…

    Promoter

    hGFAP promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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