Addgene

We narrowed to 13,733 results for: 11

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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tTA_BFP

Plasmid

#58854

Purpose

Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

tTA transcription factor fused to enhanced blue fluorescent protein

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-mCherry-DD

Plasmid

#96894

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-mCherry-DD

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-C

Plasmid

#203326

Purpose

Express N-terminal mCherry-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EcMBP165-cpGFP.PPYF.pRSET

Plasmid

#33372

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

MBP based Maltose Biosensor

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-FCU1

Plasmid

#165882

Purpose

Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3B

Plasmid

#13743

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-nptII

Plasmid

#165876

Purpose

Basic G418 resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hNanogP8

Plasmid

#19776

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

NanogP8 (NANOGP8 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB

Plasmid

#15221

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB (Creb1 Rat)

Use

Tags

Gal4

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Article

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0034

Plasmid

#196178

Purpose

MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

CaMV35S::TMV::LucF::flag::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_dCas9_VP64

Plasmid

#49015

Purpose

encodes human-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (human-codon-optimized)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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