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pPLtetO-FtsZ

Plasmid

#89656

Purpose

Plasmid for titrating synthesis of FtsZ with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

GTP-binding tubulin-like cell division protein

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTon1(acgfp1)

Plasmid

#233648

Purpose

Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

Depositor

Takeo Kubo

Article

Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

Insert

target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'

Use

Amphibian expression, tet-on

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available Since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS5

Plasmid

#218714

Purpose

Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS5 (BBS5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA4-mRuby3

Plasmid

#184890

Purpose

Stable expression of ANXA4-mRuby3 (Annexin A4-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA4 (ANXA4 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+PIF6CreN

Plasmid

#131780

Purpose

Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PIF6CreN

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-uMASS_1

Plasmid

#209762

Purpose

Expression of uMASS_1 in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 S186D

Plasmid

#16235

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
GB1-PRD(800-868)

Plasmid

#141345

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1His6-TEV-ALIX(800-868)

Tags

GB1-His6-TEV

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.hLHX6

Plasmid

#161828

Purpose

Expresses human LHX6(LIM Homeobox 6) in mammalian cells

Depositor

Daniella Ottosson

Article

Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

Insert

human LHX6 (LHX6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AA 17-23 deletion

Promoter

PGK

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt11

Plasmid

#184512

Purpose

Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 11 (Syt11 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-GFP

Plasmid

#40123

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
MTK2_001

Plasmid

#123697

Purpose

Encodes pTRE as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pTRE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCFJ1201

Plasmid

#44483

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*/124

Plasmid

#177807

Purpose

The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*/124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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