We narrowed to 7,609 results for: trac

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Article

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-YFP-SspBx6

Plasmid

#103781

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-YFP-SspBx6

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTA231-p.Syn-PAmCherry1Hfx

Plasmid

#164661

Purpose

expression of H. volcanii codon-optimized PAmCherry1

Depositor

Anita Marchfelder

Article

Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

Insert

PAmCherry1Hfx

Use

Archaeal expression

Mutation

codon-optimization for Haloferax volcanii

Promoter

p.Syn

Available Since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-FKBP-mCherry-RAB11

Plasmid

#174622

Purpose

Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

FKBP-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

FKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pNSSCPG

Plasmid

#183187

Purpose

PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

Depositor

Hsiao-Chun Huang

Article

Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

Inserts

superfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-Fc-His

Plasmid

#72145

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pROSA26-Donor

Plasmid

#60952

Purpose

Porcine ROSA26 locus targeting

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

Porcine ROSA26 homologous ARM

Use

Gene targeting

Tags

Neo EGFP

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Article

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTBL437 AAVS1 EQR sgRNA

Plasmid

#126447

Purpose

To cut the human AAVS1 locus for integration.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human AAVS1 safe-harbor locus with NGAG PAM

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

Plasmid

#178585

Purpose

Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

DLX2 (DLX2 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

Plasmid

#173868

Purpose

Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173867

Purpose

Mammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159862

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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