We narrowed to 7,267 results for: trac

1. pLL5.0-MRLC-DD-GFP

   Plasmid

   #187260

   Purpose

   Lentiviral expression of myosin regulatory light chain-DD mutant

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   T18D, S19D

   Promoter

   5' LTR

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL5.0-MRLC-AA-GFP

   Plasmid

   #187259

   Purpose

   Lentiviral expression of myosin regulatory light chain-AA mutant

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   T18A,S19A

   Promoter

   5' LTR

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. BK alpha subunit

   Plasmid

   #113566

   Purpose

   mouse BK channel alpha subunit expression vector

   Depositor

   Robert Brenner

   Article

   Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

   Insert

   Kcnma1 (Kcnma1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDG458 V3

   Plasmid

   #226959

   Purpose

   CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HCMVgH-8his (Merlin)

   Plasmid

   #136447

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-716). Codon-optimize…

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9(D10A)

   Plasmid

   #74495

   Purpose

   Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   Cas9 D10A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D10A

   Promoter

   pTeto

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-Soma-jGCaMP8s

   Plasmid

   #169256

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI-N3ICD

   Plasmid

   #185524

   Purpose

   Constitutive overexpression of human NOTCH3 intracellular domain

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH3 intracellular domain (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV mKeima-LC3

   Plasmid

   #189006

   Purpose

   Fluorescent reporter for autophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   mKeima, LC3 (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-C-prM-E-NS1

    Plasmid

    #175281

    Purpose

    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    

    Use

    Lentiviral

    Tags

    IRES EGFP

    Expression

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaCas9v2-Puro

    Plasmid

    #178802

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Use

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFPC1-ORAI1-EC-HA

    Plasmid

    #174335

    Purpose

    encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Tags

    GFP in the N-terminus and HA tag in the extracell…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBS1449 SED1-Lenti

    Plasmid

    #180824

    Purpose

    SED1 biosensor for stable integration and expression in mammalian cells

    Depositor

    Rajat Rohatgi

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 osmosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hSyn-Axon-jGCaMP8m

    Plasmid

    #172719

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. aMHC-mCherry-Rex-Blasticidin

    Plasmid

    #21228

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. SuExp His-Myc-mPLD3

    Plasmid

    #173853

    Purpose

    Protein expression plasmid for recombinant His-Myc tag mouse PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (Pld3 Mouse)
    

    Use

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTT3 Unc5A-FLAG

    Plasmid

    #72194

    Purpose

    Expresses full-length Unc5a with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a (Unc5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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