167,092 results

1. pre_IV_CS10_1545_v1

   Plasmid

   #126861

   Purpose

   4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1545_v1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1271

   Plasmid

   #143780

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HLTF (HLTF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-ncpGCaMP6s

   Plasmid

   #113674

   Purpose

   A topological variant of GCaMP6s

   Depositor

   Robert Campbell

   Article

   Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

   Insert

   ncpGCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMG059 MBP-CREB-His

   Plasmid

   #154076

   Purpose

   Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-hPPP3CB-FLAG

   Plasmid

   #179135

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-MinE-IRES-Blast

   Plasmid

   #213115

   Purpose

   Expression of untagged MinE in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE (minE )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

   Plasmid

   #79671

   Purpose

   Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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