172,029 results

1. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Traptavidin
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-zeo

   Plasmid

   #160091

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. UKNP1.9.1

   Plasmid

   #97374

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19-TdTwt

   Plasmid

   #207466

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTwt (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lamp1-mTurquoise2

   Plasmid

   #98828

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Lamp1
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-puro GFP-p62

    Plasmid

    #38277

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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