We narrowed to 24,728 results for: crispr cas9

1. A3H HapII-Cas9n-UGI

   Plasmid

   #119141

   Purpose

   Base editor made from APOBEC3H Haplotype II

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. A3H HapI-Cas9n-UGI

   Plasmid

   #119140

   Purpose

   Base editor made from APOBEC3H Haplotype I

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype I-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. dSpyCas9-E566-GS-LOV2-VPR

   Plasmid

   #231576

   Purpose

   Potent light-deactivated CRISPRa

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   dSpyCas9-VPR with an AsLOV2 insertion behind E566
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

   Plasmid

   #148849

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

   Plasmid

   #148850

   Purpose

   Mammalian Expression of HsTNRC6B-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6B-sgRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

   Plasmid

   #148852

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA2

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

   Plasmid

   #148853

   Purpose

   Mammalian Expression of HsTNRC6B-sgRNA3

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6B-sgRNA3
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

    Plasmid

    #148857

    Purpose

    Mammalian Expression of HsPatL1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsPatL1-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

    Plasmid

    #148858

    Purpose

    Mammalian Expression of HsTNRc6C-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRc6C-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid

    #172293

    Purpose

    In vitro transcription of Cas9-T2A-mClover3 synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    Cas9-T2A-mClover3
    

    Use

    CRISPR

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

    Plasmid

    #240531

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-ZIM3
    

    Tags

    FLAG, HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid

    #90085

    Purpose

    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

    Depositor

    Raffaella Sordella

    Article

    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized Domain and Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-MMLVgag-D3A-3L-dCas9-KOX1

    Plasmid

    #240532

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-KOX1
    

    Tags

    FLAG, HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

    Plasmid

    #160626

    Purpose

    TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-dCas9:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

    Plasmid

    #160623

    Purpose

    TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:dCas9:VPR-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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