We narrowed to 6,235 results for: nls

1. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-LSSmOrange-CNA35

   Plasmid

   #61605

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   LSSmOrange and CNA35
   

   Tags

   His tag

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nme2Cas9_pCDest

   Plasmid

   #119923

   Purpose

   Nme2Cas9 CMV-driven mammalian expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Tags

   NLS-3xHA-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Nuc-ExRai-AktAR2

   Plasmid

   #184049

   Purpose

   Nuclear-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Nuc-ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIA22

   Plasmid

   #214448

   Purpose

   Expresses BxbI-BP-NLS

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   BxbI
   

   Tags

   BP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-SaCas9-WPRE

   Plasmid

   #89996

   Purpose

   Expresses high levels of WT SaCas9 for genome editing

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SaCas9 (NEWENTRY Staphylococcus aureus)
   

   Tags

   3xHA, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1278

   Plasmid

   #111121

   Purpose

   Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   XRCC2 gRNA (XRCC2 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1279

    Plasmid

    #111122

    Purpose

    Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC2 gRNA (XRCC2 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1280

    Plasmid

    #111123

    Purpose

    Expresses XRCC3_+_104181759.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC3 gRNA (XRCC3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1281

    Plasmid

    #111124

    Purpose

    Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC3 gRNA (XRCC3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB-nCas9-PBE

    Plasmid

    #98160

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Maize

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1319

    Plasmid

    #111116

    Purpose

    Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T gRNA (UBE2T Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

17. DCIP

    Plasmid

    #193860

    Purpose

    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    NLS-mCherry-GFP1-10
    

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. VP64-dCas9

    Plasmid

    #177171

    Purpose

    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    VP64-dCas9(D10A, H840A)
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xFLag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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