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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,597 results for: kars;

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Showing: 391 - 420 of 11597 results

  8. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available Since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. psgRNA_EcoWG1

    Pooled Library
    #131625
    Purpose
    Bacterial inhibition library targeting every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library.
    Depositor
    David Bikard
    Article
    Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    May 12, 2020
    Availability
    Academic Institutions and Nonprofits only

  10. psgRNA Library

    Pooled Library
    #115927
    Purpose
    ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.
    Depositor
    David Bikard
    Article
    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    June 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  23. Ma-PylRS-lib1

    Pooled Library
    #224585
    Purpose
    Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Expression
    Bacterial
    Species
    Methanomethylophilus alvus
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 391 - 420 of 11597 results