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pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

Plasmid

#201217

Purpose

pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag and SNAP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCM5.66

Plasmid

#19370

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pos-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCM5.60

Plasmid

#19374

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pLOX-CWgfp

Plasmid

#12241

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of GFP. LoxP in 3' …

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

V266E

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_tdTFlag

Plasmid

#46024

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

tdTomato

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK SGK 3'UTR

Plasmid

#19865

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

SGK1 3'UTR (SGK1 Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCM5.49

Plasmid

#21391

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pal-1 3'utr in pDONRP2RP3

Use

Gateway Cloning

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.32

Plasmid

#19397

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mes-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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KHBD00716

Plasmid

#39744

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17228 (pros Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Munc18cF119E-pDEST27

Plasmid

#85625

Purpose

GST tagged Munc18c with a F119E mutation

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

F119E

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK SIRT4 3'UTR

Plasmid

#19860

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

SIRT4 3' UTR (SIRT4 Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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xfz3N/pCS2+

Plasmid

#16328

Depositor

Peter Klein

Article

Deardorff et al Development. 2001 Oct . 128(19):3655-63.

Insert

frizzled-3 (fzd3.L Frog)

Use

Vertebrate expression

Mutation

Extracellular ligand binding domain of Xenopus fr…

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM328

Plasmid

#134243

Purpose

Mammalian expression construct expressing sgRNA against PLK1

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

gRNA targeting PLK1 3'

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTW065

Plasmid

#115952

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 3'UTR Dest

Use

Synthetic Biology

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK c2orf42 3'UTR

Plasmid

#19862

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

c2orf42 3'UTR (C2orf42 Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
SSH-3 WT

Plasmid

#245957

Purpose

Expression of wild-type slingshot 3, a cofilin pSer3 phosphatase

Depositor

James Bamburg, Kensaku Mizuno, Kazumasa Ohashi

Article

Ohta et al Genes Cells. 2003 Oct;8(10):811-24. doi:

Insert

Slingshot protein phosphatase 3 (Ssh3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00630

Plasmid

#39700

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15269 (CG15269 Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
mPlum-Mito-3

Plasmid

#55988

Purpose

Localization: Mitochondria, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt3

Plasmid

#42276

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt3 (Wnt3 Mouse)

Expression

Mammalian

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
peCALWY-3

Plasmid

#22235

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

Similar to eCALWY-1, but with a linkerlength of 3…

Available Since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00397

Plasmid

#34508

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5714 (ecd Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pT7-V5-SBP-C1-HsPAN3_M

Plasmid

#146894

Purpose

Mammalian Expression of HsPAN3

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN3 (PAN3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNHA-C1-HsPAN3_M

Plasmid

#146942

Purpose

Mammalian Expression of HsPAN3

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN3 (PAN3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW328

Plasmid

#115959

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P3 With E1-3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW094

Plasmid

#115937

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 E1-3'UTR End

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CD2AP-1/3

Plasmid

#91398

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-1/3 (CD2AP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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