We narrowed to 13,503 results for: 109

1. pLG1-puro Non-targeting sgRNA 1

   Plasmid

   #109002

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_ALDOA_Glycolytic

   Plasmid

   #109854

   Purpose

   Protein expression and purification of ALDOA_Glycolytic

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ALDOA_Glycolytic (ALDOA Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. actc1b-mKate2-FAPP1

   Plasmid

   #109503

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-FAPP1
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 SWS cone opsin

   Plasmid

   #109363

   Purpose

   Human SWS cone opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   SWS cone opsin (OPN1SW Human)
   

   Tags

   1D4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_THRA_Hormone-recep

   Plasmid

   #109810

   Purpose

   Protein expression and purification of THRA_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   THRA_Hormone-recep (THRA Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST- IP2.0(codon for C.elegans)

   Plasmid

   #109216

   Purpose

   IP2.0 for C. elegans (Codon optimization and introns).

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Tags

   His-tag

   Expression

   Worm

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. actc1b-mKate2-2xFYVEhgs

   Plasmid

   #109505

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-2xFYVEhgs
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB31 (hANGPTL8-V5-His lenti)

   Plasmid

   #109116

   Purpose

   V5 and His-tagged human ANGPTL8 in a lentiviral vector

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL8 (ANGPTL8 Human)
   

   Use

   Lentiviral

   Tags

   6x His and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSe_1

   Plasmid

   #109233

   Purpose

   Empty AmpR pSC101 vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM705 Gst (Cys)

    Plasmid

    #109376

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_EEF1A2_GTP-EFTU

    Plasmid

    #109862

    Purpose

    Protein expression and purification of EEF1A2_GTP-EFTU

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    EEF1A2_GTP-EFTU (EEF1A2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1276 pSG5L HA PTEN C124S A4

    Plasmid

    #10949

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C124S; A4: S380A, T382A, T383A, S385A

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. actc1b-mKate2-rab32a

    Plasmid

    #109621

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab32a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab32a (rab32a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUM-Kan-MCS3

    Plasmid

    #109287

    Purpose

    Expresses proteins in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    New promoter/cloning site

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_PPP1CA_Metallophos

    Plasmid

    #109934

    Purpose

    Protein expression and purification of PPP1CA_Metallophos

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PPP1CA_Metallophos
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 883 pET15b PTEN

    Plasmid

    #10936

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. actc1b-mKate2-rab14

    Plasmid

    #109600

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab14

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab14 (rab14 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. actc1b-mKate2-DysF

    Plasmid

    #109492

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dysferlin

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    DysF (dysf Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-VHH-tev-std

    Plasmid

    #109423

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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