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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 261 - 280 of 11947 results

  1. ARF1 (ARF1A-c001)

    Plasmid
    #110258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ARF1 (ARF1 Human)
    Tags
    StrepII-TEV
    Expression
    Bacterial
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110258-plasmid-map-sequence-id-212329

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  12. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110626-plasmid-map-sequence-id-208002

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  15. EYFP-Talin1-FERM

    Plasmid
    #110565
    Purpose
    bacterial expresion of EYFP-tagged Talin 1
    Depositor
    Tina Izard
    Insert
    Talin 1 (Tln1 Mouse)
    Tags
    (His)6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    110565-plasmid-map-sequence-id-213906

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  17. MAEBL-COMP-blac-flag-his

    Plasmid
    #110963
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110963-plasmid-map-sequence-id-223595

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  19. KLHL3 (KLHL3A-c003)

    Plasmid
    #110251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL3 (KLHL3 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110251-plasmid-map-sequence-id-212338

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  20. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

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Showing: 261 - 280 of 11947 results