Addgene

We narrowed to 3,127 results for: RFP

Showing: 261 - 280 of 3127 results

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pL-CRISPR.EFS.tRFP

Plasmid

#57819

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTagRFP658-N1

Plasmid

#178974

Purpose

Expresses TagRFP658 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658

Use

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP713-N1

Plasmid

#136559

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTagRFP675-N1

Plasmid

#44275

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Use

Tags

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pmRFP_LAP2beta_IRES_puro2b

Plasmid

#21047

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

LAP2_beta (TMPO Rat)

Use

Tags

mRFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 14, 2009

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

PoxyR, PoxyS

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

pmRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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piRFP720-N1

Plasmid

#45461

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKTrfp

Plasmid

#59772

Purpose

pKT230 derivative; PBAD_rfp; cmR

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBADTrfp

Plasmid

#99382

Purpose

This promoter system provided the highest RFP expression upon induction

Depositor

Nathan Hillson

Insert

RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE_hygro_mRFP1_NRF2

Plasmid

#136579

Purpose

mammalian expression of mRFP1-tagged human NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTO149_RFP_CauNEO

Plasmid

#177277

Purpose

Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

RFP, CauNEO

Use

Tags

ENO1-RFP

Expression

Bacterial

Mutation

All CUG Leucine codons were replaced with the syn…

Promoter

AgTEF2

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-C1

Plasmid

#84355

Purpose

Expresses mCyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1

Use

Tags

Expression

Mammalian

Mutation

A161K and N41A, reletive to CyRFP1

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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piRFP682-N1

Plasmid

#45459

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pmiRFP709-N1

Plasmid

#79989

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTagRFP657-C1

Plasmid

#31872

Purpose

For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

Depositor

Vladislav Verkhusha

Article

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Insert

TagRFP657

Use

Tags

Expression

Mammalian

Mutation

M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Use

Tags

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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