We narrowed to 24,078 results for: Spr

1. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23-Fosprom

   Plasmid

   #188113

   Purpose

   Expresses luciferase under control of Fos promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Fos promoter (Fos Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Fos endogenous promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ166-SpRY

   Plasmid

   #161520

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9
   

   Use

   CRISPR; Gateway compatible zsprycas9 entry clone

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgDHODH-4

   Plasmid

   #186022

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pV1393:SpRY

   Plasmid

   #172189

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLCRISPR/Cas9pUbi-N

   Plasmid

   #106331

   Purpose

   Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   ZmUbi

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRainbow-DONOR1

   Plasmid

   #75398

   Purpose

   CRISPRainbow-DONOR1

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   Cm(R)-CcdB
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mutation

   Promoter

   Bacteria

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2-sgDHODH-2

   Plasmid

   #186023

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB301TOR-CRISPR

    Plasmid

    #164797

    Purpose

    For gene editing in oomycetes

    Depositor

    Miaoying Tian

    Article

    Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

    Inserts

    PsNLS-hSpCas9
    sgRNA expression cassette
    

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    HAM34 and RPL41

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR V2 sgIREB2_1

    Plasmid

    #169894

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-sgACSL4

    Plasmid

    #186024

    Purpose

    knock out ACSL4 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCRISPRed pRSETB

    Plasmid

    #159454

    Purpose

    Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Use

    Tags

    10 x His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgPBRM1-1- LentiCRISPRv2

    Plasmid

    #107403

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgPBRM1-2- LentiCRISPRv2

    Plasmid

    #107404

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPReporter-mCherry

    Plasmid

    #65008

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    User-defined

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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