Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 25,706 results for: Spr

Showing: 261 - 280 of 25706 results

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LentiCRISPR v2 hBAK

Plasmid

#129579

Purpose

CRISPR deletion of human BAK

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAK (BAK1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available Since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 hBAX

Plasmid

#129580

Purpose

CRISPR deletion of human BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAX (BAX Human)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgBAK1

Plasmid

#211520

Purpose

Deletes BAK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgBAK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pV1093:SpRY

Plasmid

#172188

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGLS_2

Plasmid

#163459

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Beclin1

Plasmid

#99574

Purpose

Expressed Cas9 with sgRNA targeting Beclin1

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting Beclin1 (BECN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGLS_6

Plasmid

#163460

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 6 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 mBAX

Plasmid

#129577

Purpose

CRISPR deletion of murine BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAX (Bax Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 mBAK

Plasmid

#129578

Purpose

CRISPR deletion of murine BAK

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAK (Bak1 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgNRF2-3

Plasmid

#186838

Purpose

knock out NRF2 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

CRISPR and Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 sgCGAS_1

Plasmid

#241454

Purpose

Delete CGAS

Depositor

Richard Possemato

Insert

sgCGAS_1 (CGAS Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgCASP8

Plasmid

#211519

Purpose

Deletes CASP8

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP8

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgNRF2-4

Plasmid

#186839

Purpose

knock out NRF2 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

CRISPR and Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgACSL4

Plasmid

#186024

Purpose

knock out ACSL4 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_hMSH2

Plasmid

#186155

Purpose

sgRNA targeting human MSH2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

MSH2 (MSH2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDHODH-4

Plasmid

#186022

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgPnpt1-2#

Plasmid

#234770

Purpose

knockout Pnpt1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Pnpt1 (Pnpt1 )

Use

Lentiviral

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 261 - 280 of 25706 results

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