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Plasmid#192058PurposeExpression vector for SOX (ORF37) R139ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutationR139APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX Q129H
Plasmid#192057PurposeExpression vector for SOX (ORF37) Q129HDepositorInsertSOX (ORF37) Q129H
UseTagsExpressionInsectMutationQ129HPromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_W1-L-W2-HECT
Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6 H216A
Plasmid#224351PurposeExpresses human KDAC6 (HDAC6) H216A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 216 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC6 H611A
Plasmid#224352PurposeExpresses human KDAC6 (HDAC6) H611A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 611 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-GST-TBK1
Plasmid#221331PurposeTBK1 protein overexpression in Sf9 insect cellsDepositorInsertTBK1 (Tbk1 Mouse)
UseTags6xHis-TEV-2xGST-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-Strep
Plasmid#211446Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionInsectMutationPromoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-FLAG
Plasmid#211447Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-TagDepositorInsertC17orf53 (HROB Human)
UseTagsFLAG-TagExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.1
Plasmid#196687PurposeRep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationTDALTTK insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.4
Plasmid#196690PurposeRep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationVVSDYTV insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.3
Plasmid#196689PurposeRep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRVDPSGL insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.2
Plasmid#196688PurposeRep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationSTIPTMK insert between amino acids 588 and 589 of…Promoterp41Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FAK W266A
Plasmid#186144PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6ExpressionInsectMutationDNA mutation T796G;G797C Amino acid mutation W266APromoterpolyhedrin promoterAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG660 lyn11-pFAST
Plasmid#172863Purposeexpresses lyn11-pFAST in mammalian cells (inner membrane labeling)DepositorInsertLyn11-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only