We narrowed to 3,935 results for: gast

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pUASp YFP Cdc42 CA

Plasmid

#52250

Purpose

Drosophila expression of YFP-Cdc42 constitutively active mutant

Depositor

Matthew Scott

Insert

Cdc42 (Cdc42 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pTIGER_mNeonGreen::3xFLAG::dCrk

Plasmid

#131137

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

3XFLAG and mNeonGreen

Expression

Insect

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral

Plasmid

#21722

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

EGFP

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-mCherry

Plasmid

#72896

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Publication

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dmBACCS2NS-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS drosophila armadillo

Plasmid

#16829

Depositor

Eric Wieschaus

Publication

Peifer et al Cell. 1990 Dec 21. 63(6):1167-76.

Insert

armadillo (arm Fly)

Expression

Bacterial

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
lentiCRISPR v2 puro - CRBN (#2)

Plasmid

#166241

Purpose

sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

Depositor

Günter Schneider

Publication

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Publication

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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AByG

Plasmid

#111083

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Publication

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right

Expression

Insect

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

Plasmid

#69899

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HIPK3 (stw Fly, Human)

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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RCAS(A) dRhoA V14 (CT#846)

Plasmid

#13949

Depositor

Cliff Tabin

Publication

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

G14V mutation

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHACK-QF2

Plasmid

#80274

Purpose

QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFOSGE_COF4C03

Plasmid

#70961

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

His2Av (His2Av Fly)

Use

Gateway Cloning

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway Cloning

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Publication

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Expression

Insect

Available since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111546

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00322

Plasmid

#34447

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8591 (CTCF Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCS5-3HA/p14ARF

Plasmid

#78764

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Publication

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

p14ARF (CDKN2A Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article