We narrowed to 4,107 results for: gast

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pCS2MT-mbeta-catenin delta5

Plasmid

#100882

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 400-666aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-mCherry

Plasmid

#72896

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta2

Plasmid

#100879

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-276aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta1

Plasmid

#100878

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-150aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Flag-NLS-hSPDEF delta1

Plasmid

#100874

Purpose

Lentiviral expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 1-248aa

Promoter

TRE2

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta2

Plasmid

#100871

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 71-246aa

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Flag-NLS-hSPDEF delta3

Plasmid

#100876

Purpose

Lentiviral expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 243-335aa

Promoter

TRE2

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta3

Plasmid

#100872

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 244-335aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta1

Plasmid

#100870

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 1-248aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRRLsin-SV40 T antigen-IRES-mCherry

Plasmid

#58993

Purpose

lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

EcoRV site in MCS converted to BamHI site

Promoter

CMV enhancer-chicken beta actin

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-V5.hOr47a

Plasmid

#126474

Purpose

Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Insert

Or47a (Or47a Fly)

Tags

V5 tag

Expression

Mammalian

Mutation

codon optimization for H. sapiens

Promoter

CMV

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E MUT

Plasmid

#110122

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KKLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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