We narrowed to 943 results for: gcg.2

1. psbA2(noATbox)-PHLS (c)

   Plasmid

   #52308

   Purpose

   Replaces the psbA2 gene in Synechocystis with the PHLS gene

   Depositor

   Anastasios Melis

   Article

   Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

   Inserts

   beta-phellandrene synthase
   Chloramphenicol resistance
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   CAAATACA box in the psbA2 promoter to ggcgcgcc

   Promoter

   psbA2

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-trmI

   Plasmid

   #89955

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   trmI gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX462-hPRKAA2-gRNA_A

   Plasmid

   #74376

   Purpose

   gRNA_A to knockout human AMPK alpha 2 using Cas9n.

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2-sgLKB1 clone3

   Plasmid

   #162127

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-sgRNA_Ago1_3

   Plasmid

   #73535

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pLVUTHshGATA1-tTR-KRAB

   Plasmid

   #11650

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

   Plasmid

   #105041

   Purpose

   Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Rictor (Rictor Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W

    Plasmid

    #105015

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    gRNA targeting Tcf7l1 (Tcf7l1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189947

    Purpose

    Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Banshee-ShCit-B

    Plasmid

    #85217

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-B
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Grin1 gRNA#1

    Plasmid

    #169789

    Purpose

    Cas9-mediated knockout of Grin1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Expression

    Mammalian

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. OA-1045D

    Plasmid

    #125006

    Purpose

    Expresses gRNAs targeting hedgehog and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    wg gRNA (wg Fly)
    

    Expression

    Insect

    Promoter

    u6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-U6-L101_guide-CBh-hSpCas9

    Plasmid

    #188547

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

    Plasmid

    #105035

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSico_U6-PLOD2 sgRNA5

    Plasmid

    #136460

    Purpose

    Expression of gRNA against human PLOD2

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    gRNA against human PLOD2 (PLOD2 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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