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Plasmid#216360PurposexhNup93-Nb4i (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb4i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC129
Plasmid#216362PurposexhNup98-Nb2i Nanobody productionDepositorInsertxhNup98-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC264
Plasmid#216363PurposexhNup98-Nb2i Nanobody productionDepositorInsertxhNup98-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-Avi-SUMOEu1ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04477
Plasmid#216352PurposexhNup35-Nb1t Nanobody productionDepositorInsertxhNup35-Nb1t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINOH11-A10
Plasmid#184278PurposeExpresses nanobody H11-A10 in E. coliDepositorInsertNanobody H11-A10
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11-B5
Plasmid#184279PurposeExpresses nanobody H11-B5 in E. coliDepositorInsertNanobody H11-B5
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_11-H6
Plasmid#184280PurposeExpresses nanobody H11-H6 in E. coliDepositorInsertNanobody H11-H6
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINH11-H4 Q98R_H100E
Plasmid#184281PurposeExpresses nanobody H11-H4 Q98R_H100Ein E. coliDepositorInsertNanobody H11-H4 Q98R_H100E
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)
Viral Prep#75470-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW (AAV1)
Viral Prep#75469-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-Flp-DOG-NW (#75469). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flp-DOG-NW plasmid DNA. EF1a-driven expression of Flp recombinase dependent on GFP (Flp-DOG); to be coinjected with GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aTagsNoneAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB_IRES_FKBP(I)-GBPen
Plasmid#128267PurposeBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).DepositorInsertsMitoTrap
FKBP(I)-GBPen
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-NBL10
Plasmid#69971PurposeCre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusionDepositorInsertNBL10
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB_IRES_FKBP(III)-GBPen
Plasmid#128268PurposeBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).DepositorInsertsMitoTrap
FKBP(III)-GBPen
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA
Plasmid#168101PurposeGFP and Flag tagged Nup62 with EPEA nanobody tagDepositorInsertGFP-Flag-Nup62-EPEA
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98
Plasmid#130704PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-F98
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only