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We narrowed to 17,151 results for: nes

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Showing: 261 - 280 of 17151 results

  5. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid
    #174081
    Purpose
    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
    Depositor
    Ryan Mehl
    Publication
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    Tags
    NES-Flag
    Expression
    Mammalian
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174081-plasmid-map-sequence-id-345715

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  7. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Publication
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  8. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid
    #173866
    Purpose
    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173866-plasmid-map-sequence-id-341714

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  9. mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid
    #173865
    Purpose
    Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173865-plasmid-map-sequence-id-341712

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  10. PB-NES-ZapCV2-Zinc dead

    Plasmid
    #203850
    Purpose
    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    Nuclear Export Signal
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203850-plasmid-map-sequence-id-402879

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  11. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid
    #118407
    Purpose
    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR-NES
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118407-plasmid-map-sequence-id-230143

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  12. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPKsub(TA)-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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  13. nes714Δ1388-1507tk/lacZ

    Plasmid
    #47616
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Publication
    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Mutation
    714 bp from 3' end of 2nd intron with a dele…
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 261 - 280 of 17151 results