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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Publication

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Snail (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled library

#83978

Purpose

Version 2 of the human CRISPRa genome wide library

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles
pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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YAP1 (S6A) - V5 in pLX304

Plasmid

#42562

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Publication

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Tags

MYC

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Publication

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Publication

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-VCPIP1

Plasmid

#22592

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

VCPIP1 (VCPIP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-HA::UPRT

Plasmid

#47110

Purpose

for subcloning UPRT into expression vectors

Depositor

Mike Cleary, Chris Doe

Publication

Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

Insert

uracil phosphoribosyltransferase

Tags

HA

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pMXs-IP spGFP-ERGIC53

Plasmid

#38270

Depositor

Noboru Mizushima

Publication

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

ERGIC-53 (LMAN1 Human)

Use

Retroviral

Tags

EGFP with Calreticulin signal peptide at N-termin…

Expression

Mammalian

Mutation

deleted signal peptide (1-30 amino acids)

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ClonTracer Barcoding Library (2.5 ug)

Pooled library

#67267

Purpose

High complexity 30bp-long semi random DNA barcode library in lentiviral backbone

Depositor

Frank Stegmeier

Publication

Bhang et al Nat Med. 2015 Apr 13. doi: 10.1038/nm.38

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

Plasmid

#104495

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

AAV1, AAV PHP.eB, and AAV Retrograde

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pcDNA3.1-ACE2-GFP

Plasmid

#154962

Purpose

pcDNA3.1-ACE2-GFP

Depositor

Utpal Pajvani

Publication

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

ACE2-GFP (ACE2 Human)

Tags

EGFP

Expression

Mammalian

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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