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pGL3-FLIP pr5

Plasmid

#19130

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +28…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pLJM1

Plasmid

#46336

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pENTR1A hu_ncOGT WT (open N-term)

Plasmid

#155197

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway cloning

Mutation

WT OGT with no start codon, in-frame with gateway…

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pDEST15.1-hRPN13 (285-386)

Plasmid

#61936

Purpose

Expression of hRPN13 (285-386) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-386) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, stop codon intro…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
HOXA10 (human) bluescript

Plasmid

#8617

Depositor

Corey Largman

Article

Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

Insert

HOXA10 (HOXA10 Human)

Expression

Bacterial

Mutation

improved Ribosomal binding site (NcoI site) at AT…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
delNC C-His Ana GvpC in pET28a

Plasmid

#85736

Purpose

Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (truncated and his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Deletion of N and C terminal regions, addition of…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pCMV

Plasmid

#8815

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-5-4a

Plasmid

#40045

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-5-4a (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Slp4 linker substituted by Slp5 linker domain (s…

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

Plasmid

#236148

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the UTRN promoter region

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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SCN1Aprom(335-406)-H2B-GFP

Plasmid

#236151

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the SCN1a promoter regi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23-WIPI1promotor

Plasmid

#206840

Purpose

The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

WIPI1 promotor (partial)

Use

Luciferase

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB55 - pL0_CrGLK (CDS)

Plasmid

#203903

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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p2702-AGER-gRNA1

Plasmid

#216471

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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