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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Publication

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

John Doench, David Root

Publication

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Publication

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Tags

GFP and His

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Beclin1

Plasmid

#99574

Purpose

Expressed Cas9 with sgRNA targeting Beclin1

Depositor

Edward Campbell

Publication

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting Beclin1 (BECN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQTEV-STX6

Plasmid

#31581

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

syntaxin 6 (STX6 Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Publication

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WHaloCaMP1a-EGFP

Expression

Mammalian

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Publication

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Tags

GST and PSP

Expression

Bacterial

Promoter

tac

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Publication

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Expression

Bacterial

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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Bsrs078-LasR

Plasmid

#85147

Purpose

Constitutively expresses LasR

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Insert

LasR

Available since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

Plasmid

#24584

Depositor

Thomas Wandless

Publication

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Publication

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDsRed-miR9 Sensor

Plasmid

#22742

Depositor

Lynn Hudson

Publication

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9 binding sites

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Publication

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EB1 (MAPRE1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEYFP(C1)-PP1alpha

Plasmid

#44231

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP1A (PPP1CA Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-synapsin-HaloCaMP1b-EGFP

Plasmid

#138328

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Publication

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has service

AAV1 and AAV9

Insert

HaloCaMP1b

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Promoter

synapsin

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Publication

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Tags

NLS-3xHA

Expression

Insect

Promoter

act5C

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-FGFR4

Plasmid

#60531

Purpose

Entry vector for human FGFR4

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

FGFR4 (FGFR4 Human)

Use

Gateway Cloning

Mutation

c2134a P712T

Promoter

NA

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCMU-LEr

Plasmid

#61185

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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