We narrowed to 26,550 results for: cat

1. pDisplay-GACh1.0

   Plasmid

   #106072

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   6-His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECE HA AMPKa1 AS

   Plasmid

   #69831

   Purpose

   Expresses Expresses AMPK alpha 1 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-CTSL

   Plasmid

   #172414

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Procathepsin L (Cathepsin L1) (CTSL Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTag-RFP-C-h-Rab4a-c-Myc

   Plasmid

   #79800

   Purpose

   Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab4a (RAB4A Human)
   

   Use

   Tags

   TagRFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAMKIIa

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC/CMV-JAK1P1084A-VSV

    Plasmid

    #139358

    Purpose

    Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    P1084A

    Promoter

    CMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

    Plasmid

    #16752

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before DEP (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before DEP

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-Caveolin3DGV

    Plasmid

    #67886

    Purpose

    Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

    Depositor

    Rob Parton

    Article

    Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

    Insert

    Cav3 (Cav3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 53 amino acids of Caveolin3

    Promoter

    CMV

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

18. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. shYES1 # 1

    Plasmid

    #42546

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. bu6-sgCebpa_v2-mU6-sgCebpb_v2-hU6-sgCebpd_v2

    Plasmid

    #177258

    Purpose

    Expresses Cebpa_v2 (bU6), Cebpb_v2 (mU6), Cebpd_v2 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v2/sgCebpb_v2/sgCebpd_v2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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