We narrowed to 6,919 results for: dna plasmids

1. Bxb1-GA_AIO-TetOn_Divergent Donor

   Plasmid

   #229786

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelp

   Plasmid

   #227583

   Purpose

   Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   guide RNA scaffold
   

   Use

   Cloning vector

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. rs214-minor

   Plasmid

   #113380

   Purpose

   evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   rs2149943 minor allele
   

   Use

   Luciferase

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-SpyCatcher

   Plasmid

   #79053

   Purpose

   Expresses maltose binding protein (MBP) SpyCatcher fusion

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   MBP-SpyCatcher
   

   Tags

   6xHis and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Derived from pDEST14-SpyCatcher (Plasmid #35044)…

   Promoter

   T7

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmEos3.2:cat

   Plasmid

   #78100

   Purpose

   Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

   Depositor

   Peng Chen

   Article

   Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

   Insert

   mEos3.2-cat
   

   Expression

   Bacterial

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Bxb1-GT_CAG_3xTurboGFP-NLS

   Plasmid

   #229777

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   3x TurboGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1-GA_CAG-TurboGFP

   Plasmid

   #229781

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TurboGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AX53_pS.Donor.S1.TS

   Plasmid

   #100290

   Purpose

   AAVS1-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1-a and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKB01-PssbB-mKate2

    Plasmid

    #69105

    Purpose

    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mKate2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC31

    Plasmid

    #209996

    Purpose

    Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C2SN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC32

    Plasmid

    #209997

    Purpose

    Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C5CSN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC52

    Plasmid

    #209991

    Purpose

    Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C2SN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC51

    Plasmid

    #209990

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C1CSN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AV09_pDonor.EP.S1.TS

    Plasmid

    #100297

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AY42_pS.Donor.R5

    Plasmid

    #100291

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF256

    Plasmid

    #105496

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF257

    Plasmid

    #105497

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRenilla (GB0056)

    Plasmid

    #68198

    Purpose

    Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla coding region
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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