We narrowed to 4,933 results for: gne

1. hKCNQ5(S448I).ires.GFP-pcDNA3

   Plasmid

   #204371

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S448I

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hKCNQ5(R178X).ires.GFP-pcDNA3

   Plasmid

   #204372

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R178X

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hKCNQ5(R443X).ires.GFP-pcDNA3

   Plasmid

   #204373

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R443X

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3. Flag-CSL Q307R

   Plasmid

   #167009

   Purpose

   Mammalian expression of CSL Q307R

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q307R

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3. Flag-CSL A258R

   Plasmid

   #167008

   Purpose

   Mammalian expression of CSL A258R

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A258R

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3. Flag-CSL V237A

   Plasmid

   #167007

   Purpose

   Mammalian expression of CSL V237A

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V237A

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3. Flag-CSL F235R

   Plasmid

   #167006

   Purpose

   Mammalian expression of CSL F235R

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F235

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-Basic-ZC3H12D_PromoterMUT

   Plasmid

   #153067

   Purpose

   ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

   Promoter

   None

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-GST-TEV-BRD4(42-459) BD1-BD2

    Plasmid

    #233887

    Purpose

    pGex for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB2

    Plasmid

    #195282

    Purpose

    Bicistronic DTR and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Puro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-N1_c-SRC

    Plasmid

    #171850

    Purpose

    pEGFP-N1_c-SRC

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    c-SRC (SRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Added a linker sequence in 3' of c-SRC: GSGS

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB-EPCP1

    Plasmid

    #226783

    Purpose

    Expression of Z-tagged LRRK2 RCKW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1326

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5-LRRK2

    Plasmid

    #229019

    Purpose

    Expression of untagged full length human LRRK2 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    no tags (untagged)

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1 Sox2 3H b

    Plasmid

    #26352

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. GFP-KIF1B beta-600-1400

    Plasmid

    #79109

    Purpose

    Mammalian expression of GFP-KIF1B beta-600-1400

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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