We narrowed to 8,953 results for: plat

1. ET8-GPIba-6His

   Plasmid

   #102878

   Purpose

   Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus

   Depositor

   Timothy Springer

   Article

   Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1

   Insert

   Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
   

   Tags

   6xHis tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121817

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS-mTagRFP-TUBA1B

   Plasmid

   #62850

   Purpose

   Donor vector for gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   mTagRFP-TUBA1B (TUBA1B Human, Synthetic)
   

   Use

   Yeast-e.coli shuttle vector

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. F2R-bio-His

   Plasmid

   #51863

   Purpose

   Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F2R (F2R Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121814

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-his-Gluc-iLID

   Plasmid

   #172096

   Purpose

   Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - iLID
   

   Expression

   Bacterial

   Mutation

   Gluc Mutations - M43L, M110L

   Promoter

   T7

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_GFP_Luciferase

   Plasmid

   #155079

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

   Plasmid

   #121841

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TTMP-His-bio

    Plasmid

    #52335

    Purpose

    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TTMP (C3orf52 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid

    #207768

    Purpose

    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. INSR-bio-His

    Plasmid

    #53341

    Purpose

    Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    INSR (INSR Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB-GFP-Dnmt3b6-Poly(A)

    Plasmid

    #65532

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid

    #155059

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121816

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Gluc-EL222-his

    Plasmid

    #172097

    Purpose

    Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - EL222
    

    Expression

    Bacterial

    Mutation

    Gluc mutations - M43L, M110L

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CFP-GIV-CT FA

    Plasmid

    #69533

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV w…

    Promoter

    CMV

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCHKO_PDPR_exon_deletion_1

    Plasmid

    #155065

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CFP-GIV-CT WT

    Plasmid

    #69532

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV

    Promoter

    CMV

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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