We narrowed to 54,133 results for: tra

1. pMS24: pCascade(PmcCAST)_entry

   Plasmid

   #168157

   Purpose

   Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-24p3/LCN2-282kB mut-LUC

   Plasmid

   #46867

   Purpose

   murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   NF- kB site is mutated (see comments)

   Promoter

   24p3 promoter (inactive)

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pGL3-24p3/LCN2-282C/EBP mut-LUC

   Plasmid

   #46868

   Purpose

   murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   CEBP site is mutated (see comments)

   Promoter

   24p3 promoter (inactive)

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

   Plasmid

   #174639

   Purpose

   Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-GFP-ppKinVIb(861-1321)
   

   Use

   Tags

   SSPB(micro)-GFP

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

   Plasmid

   #182354

   Purpose

   Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

   Plasmid

   #182356

   Purpose

   Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB165 - pL1R3_pMAS::rLUC-I::tMAS

   Plasmid

   #123198

   Purpose

   binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   pMAS::rLUC-I::tMAS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tmem38A mRFP-N2 (800)

   Plasmid

   #62007

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem38A (TMEM38A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. WFS1 mRFP-N2 (891)

   Plasmid

   #62018

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   WFS1 (WFS1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. TAPBPL pEGFP-C3 (1765)

    Plasmid

    #62055

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    TAPBPL (TAPBPL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAV10.F3

    Plasmid

    #63199

    Purpose

    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Nanog-t2A-mCherry

    Plasmid

    #127541

    Purpose

    Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codes for the last 110 a.a of Nanog

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NET4 pEGFP-N2 (1201)

    Plasmid

    #62041

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    Net4 (TMEM53 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAV113.loxP

    Plasmid

    #63186

    Purpose

    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV115.loxP

    Plasmid

    #63187

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV116.loxP

    Plasmid

    #63188

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK597

    Plasmid

    #71121

    Purpose

    Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHW1387

    Plasmid

    #229880

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    

    Use

    Tags

    GFP-C1

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHW1385

    Plasmid

    #229879

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    

    Use

    Tags

    mScarlet-I-C1

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW1392

    Plasmid

    #229878

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    

    Use

    Tags

    GFP-C1

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge