We narrowed to 7,360 results for: ust

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Flag-ZDHHC24

Plasmid

#231672

Purpose

Expresses human ZDHHC24 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC24 (ZDHHC24 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-30a-METTL17-8XHIS

Plasmid

#215705

Purpose

Express METTL17-8XHIS in C41(DE3) cells

Depositor

Vamsi Mootha

Article

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Tags

HIS

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_PCP-T5Exo

Plasmid

#214736

Purpose

encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

Depositor

Gil Westmeyer

Article

Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

Insert

CAG_PCP-T5Exo_bGH

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDual-IN-SMN1

Plasmid

#215721

Purpose

dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN1 EXON 7 (SMN1 Human)

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

Plasmid

#63931

Purpose

Empty AAV-RAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451-CMV2-mir1

Plasmid

#164635

Purpose

Express miR-451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR451a (MIR451A Human)
miR-1-1 (MIR1-1 )

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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0_T7-mScarlet3

Plasmid

#225927

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pS148∙CsR

Plasmid

#197858

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Ap/Cb resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5 (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-SV40 polyA signal

Plasmid

#48627

Purpose

Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

SV40 early polyadenylation signal cassette

Use

Gateway entry vector

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-HRD1

Plasmid

#231688

Purpose

Expresses human HRD1 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

HRD1 (SYVN1 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

Plasmid

#233080

Purpose

Expression vector for sgRNA-S1b and eCas12f1i

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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