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pHACK(Gal4)-DONR(T2A-Cas9)

Plasmid

#194768

Purpose

To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

T2A-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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p6868 MSCV-IP N-V5 Zer1

Plasmid

#35100

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

Zer1 (ZER1 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag

Plasmid

#209658

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Has Service

AAV5

Insert

cpSFGFP.HaloTag

Use

AAV

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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WNK2 K207A

Plasmid

#24570

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2` (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Mutation

K207A

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
G1BgSVNA-CDK4

Plasmid

#16573

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-tag2B-PISP/AIPP1

Plasmid

#47606

Depositor

Emanuel Strehler

Article

Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

Insert

PISP (PDZD11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pGSTag Bmf

Plasmid

#24264

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
[Bla][IRES][GFP]

Plasmid

#97193

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Prx2(C172A)

Plasmid

#158228

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2(C172A)

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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p426 25Q GAL

Plasmid

#1187

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pCAG1.1-TSSK2/3xFLAG

Plasmid

#199625

Purpose

Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk2 mRNA (Tssk2 Mouse)

Tags

3xFLAG

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Tags

Strep II-MBP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-EGFR

Plasmid

#208274

Purpose

Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCherry-EGFR

Use

Lentiviral

Tags

mCherry

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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p426 72Q GPD

Plasmid

#1183

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4

Plasmid

#220451

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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