We narrowed to 8,691 results for: BLI;

1. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIX403-EBFP2-ccdB-Puro

   Plasmid

   #158552

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-AID-EGFP

   Plasmid

   #80076

   Purpose

   Mammalian expression vector for C-terminal AID-EGFP tagging

   Depositor

   Andrew Holland

   Insert

   AID-EGFP
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-EGFP-AID

   Plasmid

   #80075

   Purpose

   Mammalian expression vector for N-terminal EGFP-AID tagging

   Depositor

   Andrew Holland

   Insert

   EGFP-AID
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-V5-SBP-C1-HshnRNPE1

   Plasmid

   #64921

   Purpose

   Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Insert

   hnRNPE1 (PCBP1 Human)
   

   Use

   Tags

   V5 and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGIG-6xHIS.Mm.RPE65-eGFP

   Plasmid

   #41019

   Depositor

   John Nickerson

   Insert

   RPE65 (Rpe65 Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-ePEG-LINE1-loxPsym

   Plasmid

   #216866

   Purpose

   Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

   Depositor

   Leopold Parts

   Insert

   spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-NT-3-mCherry

    Plasmid

    #190201

    Purpose

    To express the mouse NT-3 tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    NT-3
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

    Plasmid

    #48284

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-EGFP-DmRRP40

    Plasmid

    #65797

    Purpose

    expresses EGFP-tagged RRP40 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    RRP40 (Rrp40 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTre-Tight-luciferase-GFP (467)

    Plasmid

    #65491

    Purpose

    negative control for expression assays

    Depositor

    Roderic Guigo, Rory Johnson

    Insert

    EGFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3a-His-DJ1

    Plasmid

    #51488

    Purpose

    Inducible expression of human His-DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. mouse ptc probe M2-3

    Plasmid

    #58701

    Purpose

    makes mouse ptc probe for in situ hybridization

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Bacterial

    Mutation

    only EcoRI fragment (5' end)

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. TRIOBP-TwinStrepII

    Plasmid

    #83201

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TRIOBP (Triobp Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKSB- 3xP3-mTurqSV40 GGGG AAGA

    Plasmid

    #174533

    Purpose

    3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-mTurquoise BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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